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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
06/10/2022 |
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Actualizado : |
06/10/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GIANNITTI, F.; SILVEIRA, C.S.; BULLOCK, H.; BERON, M.; FERNÁNDEZ-CIGANDA, S.; BENÍTEZ-GALEANO, M.J.; RODRÍGUEZ-OSORIO, N.; SILVA-FLANNERY, L.; PERDOMO, T.; CABRERA, A.; PUENTES, R.; COLINA, R.; RITTER, J.M.; CASTELLS, M. |
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Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; HANNAH BULLOCK, Synergy America Inc., Atlanta, GA 30329, USA; MARINA MAURENTE BERON, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SOFÍA FERNÁNDEZ-CIGANDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Unidad de Genómica y Bioinformática, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; NÉLIDA RODRÍGUEZ-OSORIO, Unidad de Genómica y Bioinformática, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; LUCIANA SILVA-FLANNERY, Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30329, USA; TERESITA YISELL PERDOMO TORRES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRÉS CABRERA, Facultad de Veterinaria, Universidad de la República, Montevideo 13000, Uruguay; Laboratorio de Interacciones Hospedero-Patógeno, Institut Pasteur de Montevideo, Montevideo 11400, Uruguay; RODRIGO PUENTES, Facultad de Veterinaria, Universidad de la República, Montevideo 13000, Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; JANA M. RITTER, Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30329, USA; MATÍAS CASTELLS, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay. |
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Título : |
Bovine Polyomavirus-1 (Epsilonpolyomavirus bovis): An emerging fetal pathogen of cattle that causes renal lesions resembling Polyomavirus-associated nephropathy of humans. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Viruses, 2022; 14 (9): 2042. OPEN ACCESS. doi: https://doi.org/10.3390/v14092042 |
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ISSN : |
1999-4915 |
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DOI : |
10.3390/v14092042 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 12 August 2022; Revised 8 September 2022: Accepted 9 September 2022; Published 14 September 2022.
Academic Editors: Fernando Bauermann and Mayara Maggioli.
Correspondence authors: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Colonia, Uruguay; email:fgiannitti@inia.org.uy - Castells, M.; Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay; email:matiascastellsbauer@gmail.com --
Funding: Instituto Nacional de Investigación Agropecuaria (INIA), grant PL_27 N-23398. -- This article belongs to the Special Issue Pathogenesis and Host Responses to Viral Diseases in Livestock Species: https://www.mdpi.com/journal/viruses/special_issues/pathogenesis_livestock |
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Contenido : |
ABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the "cytopathic-inflammatory pathology pattern" proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing a cytopathic effect in Madin-Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed. © 2022 by the authors. MenosABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus mor... Presentar Todo |
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Palabras claves : |
Abortion; Cattle; Emerging diseases; Epsilonpolyomavirus bovis; Nephropathy; Next generation sequencing; Pathology; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; Polyomavirus; Reproductive diseases; Viral diseases. |
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Asunto categoría : |
L20 Ecología animal |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16810/1/viruses-14-02042-v2.pdf
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Marc : |
LEADER 04533naa a2200445 a 4500
001 1063641
005 2022-10-06
008 2022 bl uuuu u00u1 u #d
022 $a1999-4915
024 7 $a10.3390/v14092042$2DOI
100 1 $aGIANNITTI, F.
245 $aBovine Polyomavirus-1 (Epsilonpolyomavirus bovis)$bAn emerging fetal pathogen of cattle that causes renal lesions resembling Polyomavirus-associated nephropathy of humans.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 12 August 2022; Revised 8 September 2022: Accepted 9 September 2022; Published 14 September 2022. Academic Editors: Fernando Bauermann and Mayara Maggioli. Correspondence authors: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Colonia, Uruguay; email:fgiannitti@inia.org.uy - Castells, M.; Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay; email:matiascastellsbauer@gmail.com -- Funding: Instituto Nacional de Investigación Agropecuaria (INIA), grant PL_27 N-23398. -- This article belongs to the Special Issue Pathogenesis and Host Responses to Viral Diseases in Livestock Species: https://www.mdpi.com/journal/viruses/special_issues/pathogenesis_livestock
520 $aABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the "cytopathic-inflammatory pathology pattern" proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing a cytopathic effect in Madin-Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed. © 2022 by the authors.
653 $aAbortion
653 $aCattle
653 $aEmerging diseases
653 $aEpsilonpolyomavirus bovis
653 $aNephropathy
653 $aNext generation sequencing
653 $aPathology
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPolyomavirus
653 $aReproductive diseases
653 $aViral diseases
700 1 $aSILVEIRA, C.S.
700 1 $aBULLOCK, H.
700 1 $aBERON, M.
700 1 $aFERNÁNDEZ-CIGANDA, S.
700 1 $aBENÍTEZ-GALEANO, M.J.
700 1 $aRODRÍGUEZ-OSORIO, N.
700 1 $aSILVA-FLANNERY, L.
700 1 $aPERDOMO, T.
700 1 $aCABRERA, A.
700 1 $aPUENTES, R.
700 1 $aCOLINA, R.
700 1 $aRITTER, J.M.
700 1 $aCASTELLS, M.
773 $tViruses, 2022; 14 (9): 2042. OPEN ACCESS. doi: https://doi.org/10.3390/v14092042
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
10/05/2018 |
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Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
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Autor : |
GARCIA, C.; CARLESSO, R.; FIORIN, T.T.; DE BONA, F.D.; MELO, G.L. |
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Afiliación : |
CLAUDIO CESAR GARCIA GALLARRETA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; R. CARLESSO; T.T. FIORIN; F.D. DE BONA; G.L. MELO. |
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Título : |
Modificaçoes morfologicas das plantas de milho submetidas a diferentes manejos de água de irrigaçao. |
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Fecha de publicación : |
2002 |
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Fuente / Imprenta : |
ln: Workshop em Engenharia Agrícola (2002 : Santa Maria, RS), Mostra dos trabalhos científicos do PPGEA do ano de 2002 / Santa Maria, RS : Universidade Federal de Santa Maria, Centro de Ciências Rurais, Programa de Pós-Graduação em Engenh 2002. |
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Páginas : |
p. 148-152 |
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Idioma : |
Portugués |
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Notas : |
Publicado en Anais do XXXI Congresso Brasileiro de Engenharia Agrícola - CONBEA 2002, Salvador - BA, 29 junho a 2 de agosto de 2002 Brasil. Ministério da Educaçao; Universidade Federal de Santa María. Centro de Ciencias Rurais. Programa de Pós-Graduaçao em Engenharia Agrícola |
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Contenido : |
ABSTRACT:
The objective of this experiment was to evaluate the application of different values of maximum crop evapotranspiration as irrigation trigger on maize growth. The experiment was conducted during the 2000/2001 growing season in the experimental field of the Agricultura) Engineering Department of the Federal University of Santa Maria, RS-BRAZIL. A set of 24 drainage lysimeters was used. The experimental site was protected against rainfall by a mobile arch rainshelter. Maize plants were submitted to five irrigated treatments. Irrigation 's were applied when cumulative daily maximum .crop evapotranspiration (ETmc), estimated by the Penman-Monteith equation, reached values of 14, 22, 30, 38 and 46 mm. A completely randomized design was used with four replications. Results demonstrated that there were no differences for leaf area indet. Use of the irrigation water management of 34 mm of ETmc resulted in higher plants.
RESUMO:
O objetivo desse experimento foi avaliar modifica\:5es morfológicas de plantas de milho submetidas a diferentes manejos da água de irriga\:iio. O experimento foi conduzido no ano agrícola de 2000/2001 em área experimental do Departamento de Engenharia Rural da Universidade Federal de Santa Maria-RS. O experimento foi realizado em um conjunto de 24 lisímetros de drenagem construídos em fibra de vidro com dimens5es de 1,40 m x 0,95 me protegidos das recipita\:5es pluviais através de urna cobertura móvel de polietileno impermeável. O experimento foi conduzido no delineamento experimental inteiramente casualizado, com quatro repeti\:5es. As plantas de milho foram submetidas a cinco manejos da água de irriga\:ao. Irriga\:5es foram aplicadas sempre que a evapotranspira\:ao máxima da cultura acumulada (ETma) estimada pelo método de Penman-Monteith alcan\:ava valores de 14, 22, 30, 38 e 46 mm. Os resultados demonstraram que entre todos os manejos da água de irriga\:ao aplicados nao houve diferen\:as significativas para índice de área foliar final. O manejo da água de irrigaºªº de 34 mm de valor da ETma ocasionou mais altura de plantas de milho. MenosABSTRACT:
The objective of this experiment was to evaluate the application of different values of maximum crop evapotranspiration as irrigation trigger on maize growth. The experiment was conducted during the 2000/2001 growing season in the experimental field of the Agricultura) Engineering Department of the Federal University of Santa Maria, RS-BRAZIL. A set of 24 drainage lysimeters was used. The experimental site was protected against rainfall by a mobile arch rainshelter. Maize plants were submitted to five irrigated treatments. Irrigation 's were applied when cumulative daily maximum .crop evapotranspiration (ETmc), estimated by the Penman-Monteith equation, reached values of 14, 22, 30, 38 and 46 mm. A completely randomized design was used with four replications. Results demonstrated that there were no differences for leaf area indet. Use of the irrigation water management of 34 mm of ETmc resulted in higher plants.
RESUMO:
O objetivo desse experimento foi avaliar modifica\:5es morfológicas de plantas de milho submetidas a diferentes manejos da água de irriga\:iio. O experimento foi conduzido no ano agrícola de 2000/2001 em área experimental do Departamento de Engenharia Rural da Universidade Federal de Santa Maria-RS. O experimento foi realizado em um conjunto de 24 lisímetros de drenagem construídos em fibra de vidro com dimens5es de 1,40 m x 0,95 me protegidos das recipita\:5es pluviais através de urna cobertura móvel de polietileno impermeável. O experimento fo... Presentar Todo |
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Palabras claves : |
EVAPOTRANSPIRAÇAO; EVAPOTRANSPIRATION; ÍNDICE DE ÁREA FOLIAR; IRRIGATION MANAGEMENT; LEAF AREA INDEX; MANEJO DE IRRIGAÇAO. |
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Thesagro : |
EVAPOTRANSPIRACION; INDICE DE SUPERFICIE FOLIAR; MAIZ; MANEJO DEL SUELO; RIEGO. |
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Asunto categoría : |
P05 Gestión de recursos energéticos |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9536/1/Garcia-C.-2002.-PPGEA-2002-p.148-152.pdf
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Marc : |
LEADER 03509naa a2200325 a 4500
001 1010842
005 2018-05-10
008 2002 bl uuuu u00u1 u #d
100 1 $aGARCIA, C.
245 $aModificaçoes morfologicas das plantas de milho submetidas a diferentes manejos de água de irrigaçao.
260 $c2002
300 $ap. 148-152
500 $aPublicado en Anais do XXXI Congresso Brasileiro de Engenharia Agrícola - CONBEA 2002, Salvador - BA, 29 junho a 2 de agosto de 2002 Brasil. Ministério da Educaçao; Universidade Federal de Santa María. Centro de Ciencias Rurais. Programa de Pós-Graduaçao em Engenharia Agrícola
520 $aABSTRACT: The objective of this experiment was to evaluate the application of different values of maximum crop evapotranspiration as irrigation trigger on maize growth. The experiment was conducted during the 2000/2001 growing season in the experimental field of the Agricultura) Engineering Department of the Federal University of Santa Maria, RS-BRAZIL. A set of 24 drainage lysimeters was used. The experimental site was protected against rainfall by a mobile arch rainshelter. Maize plants were submitted to five irrigated treatments. Irrigation 's were applied when cumulative daily maximum .crop evapotranspiration (ETmc), estimated by the Penman-Monteith equation, reached values of 14, 22, 30, 38 and 46 mm. A completely randomized design was used with four replications. Results demonstrated that there were no differences for leaf area indet. Use of the irrigation water management of 34 mm of ETmc resulted in higher plants. RESUMO: O objetivo desse experimento foi avaliar modifica\:5es morfológicas de plantas de milho submetidas a diferentes manejos da água de irriga\:iio. O experimento foi conduzido no ano agrícola de 2000/2001 em área experimental do Departamento de Engenharia Rural da Universidade Federal de Santa Maria-RS. O experimento foi realizado em um conjunto de 24 lisímetros de drenagem construídos em fibra de vidro com dimens5es de 1,40 m x 0,95 me protegidos das recipita\:5es pluviais através de urna cobertura móvel de polietileno impermeável. O experimento foi conduzido no delineamento experimental inteiramente casualizado, com quatro repeti\:5es. As plantas de milho foram submetidas a cinco manejos da água de irriga\:ao. Irriga\:5es foram aplicadas sempre que a evapotranspira\:ao máxima da cultura acumulada (ETma) estimada pelo método de Penman-Monteith alcan\:ava valores de 14, 22, 30, 38 e 46 mm. Os resultados demonstraram que entre todos os manejos da água de irriga\:ao aplicados nao houve diferen\:as significativas para índice de área foliar final. O manejo da água de irrigaºªº de 34 mm de valor da ETma ocasionou mais altura de plantas de milho.
650 $aEVAPOTRANSPIRACION
650 $aINDICE DE SUPERFICIE FOLIAR
650 $aMAIZ
650 $aMANEJO DEL SUELO
650 $aRIEGO
653 $aEVAPOTRANSPIRAÇAO
653 $aEVAPOTRANSPIRATION
653 $aÍNDICE DE ÁREA FOLIAR
653 $aIRRIGATION MANAGEMENT
653 $aLEAF AREA INDEX
653 $aMANEJO DE IRRIGAÇAO
700 1 $aCARLESSO, R.
700 1 $aFIORIN, T.T.
700 1 $aDE BONA, F.D.
700 1 $aMELO, G.L.
773 $tln: Workshop em Engenharia Agrícola (2002 : Santa Maria, RS), Mostra dos trabalhos científicos do PPGEA do ano de 2002 / Santa Maria, RS : Universidade Federal de Santa Maria, Centro de Ciências Rurais, Programa de Pós-Graduação em Engenh 2002.
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